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R&D Systems anti prdm16
Anti Prdm16, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+prdm16/pm41963339-387-20-21?v=R%26D+Systems
Average 94 stars, based on 35 article reviews

anti prdm16 - by Bioz Stars, 2026-08

94/100 stars

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a A diagram shows the area of lateral V-SVZ used in the quantification and shown in ( c ). Quantification of the number of radial processes at one month of age in the striatum. Images were taken between −0.1 mm posterior and 0.74 mm anterior to Bregma. Only radial processes that extend beyond 200 μm away from the ventricular walls were counted. n = 4 mice per genotype. All statistical tests were performed using data from each mouse (biological replicate) as an individual observation in all figures except for scRNAseq. Two-tailed Welch’s t -test. p = 0.0276. In all figures: *, p < 0.05. **, p < 0.01. ***, p < 0.001. N.S. not significant. Data are presented as means ± SEM as appropriate. Created in BioRender. Zhang, Y. (2025) https://BioRender.com/y53n311 . b A diagram shows the area of the cerebral cortex used in the quantification and shown in ( d ). Quantification of the number of radial processes at P21 in the cerebral cortex. Images were taken between 0.1 and 1.18 mm anterior to Bregma. Only radial processes that extend beyond the white matter were counted. n = 3 mice per genotype. Two-tailed Welch’s t -test. p = 0.0021. Data are presented as means ± SEM as appropriate. Created in BioRender. Zhang, Y. (2025) https://BioRender.com/y53n311 . c Persistence of GFAP + /Nestin + radial processes in juvenile and adult Prdm16 cKO mice. Scale bar: 50 μm. The dashed lines indicate ventricular walls. d Persistence of GFAP + radial processes in P21 Prdm16 cKO cortex. Scale bar: 100 μm.

Journal: Nature Communications

Article Title: Prdm16 regulates the postnatal fate of embryonic radial glia via Vcam1-dependent mechanisms

doi: 10.1038/s41467-025-60895-y

Figure Lengend Snippet: a A diagram shows the area of lateral V-SVZ used in the quantification and shown in ( c ). Quantification of the number of radial processes at one month of age in the striatum. Images were taken between −0.1 mm posterior and 0.74 mm anterior to Bregma. Only radial processes that extend beyond 200 μm away from the ventricular walls were counted. n = 4 mice per genotype. All statistical tests were performed using data from each mouse (biological replicate) as an individual observation in all figures except for scRNAseq. Two-tailed Welch’s t -test. p = 0.0276. In all figures: *, p < 0.05. **, p < 0.01. ***, p < 0.001. N.S. not significant. Data are presented as means ± SEM as appropriate. Created in BioRender. Zhang, Y. (2025) https://BioRender.com/y53n311 . b A diagram shows the area of the cerebral cortex used in the quantification and shown in ( d ). Quantification of the number of radial processes at P21 in the cerebral cortex. Images were taken between 0.1 and 1.18 mm anterior to Bregma. Only radial processes that extend beyond the white matter were counted. n = 3 mice per genotype. Two-tailed Welch’s t -test. p = 0.0021. Data are presented as means ± SEM as appropriate. Created in BioRender. Zhang, Y. (2025) https://BioRender.com/y53n311 . c Persistence of GFAP + /Nestin + radial processes in juvenile and adult Prdm16 cKO mice. Scale bar: 50 μm. The dashed lines indicate ventricular walls. d Persistence of GFAP + radial processes in P21 Prdm16 cKO cortex. Scale bar: 100 μm.

Article Snippet: Brains were sectioned on a cryostat (Leica) and 30 μm floating coronal sections were blocked and permeabilized in 10% donkey serum with 0.2% Triton X-100 in PBS and then stained with primary antibodies against PRDM16 (Patrick Seale lab, dilution 1:100), PRDM16 (R&D system, AF6295-SP, 1:200), GFAP (Biolegend, 829401, 1:1500 and Dako, Z0334, 1:1500), Nestin (Aveslabs, NES-0020, 1:500), Vcam1 (BD Biosciences, 550547,1:100), DCX (Abcam, ab18723, 1:500), BrdU (Abcam, ab6326, 1:500), Parvalbumin (Novus Biologicals, NB120-11427SS, 1:500), TH (Sigma, MAB318, 1:400), Calretinin (Sigma, ZMS1073-25UL, 1:400), NeuN (AbCam, ab177487, 1:1000), Satb2 (AbCam, ab34735, 1:1000), and Foxp1 (Bennett Novitch, MI 585, 1:16000) at 4 °C overnight.

Techniques: Two Tailed Test

a Whole-mount preparation, in which the entire surface of the lateral walls of the lateral ventricles is cut out for immunohistochemistry experiments. b Neural stem cells and ependymal cells were identified by a combination of markers on whole-mount preparations. Neural stem cells are positive for Vcam1 and Sox2 with single dots positive for γ-tubulin immunofluorescence (a single basal body associated with a single primary cilium). Ependymal cells are negative for Vcam1 with clusters of γ-tubulin immunofluorescence (multiple basal bodies associated with multiple cilia). Created in BioRender. Zhang, Y. (2025) https://BioRender.com/p63y337 . c An example of a Vcam1 + neural stem cell with a single γ-tubulin + dot (arrowhead) and examples of Vcam1 − ependymal cells with clusters of γ-tubulin + dots (arrows). Scale bar: 10 μm. The experiment was repeated more than three times. d Vcam1, γ-tubulin, and Sox2 immunofluorescence on whole-mount preparations of P35 Prdm16 cKO and control mice. Scale bar: 50 μm. e Quantification of neural stem and ependymal cells at P34–35. n = 3 mice per genotype. Two-tailed Welch’s t -test, total cells p = 0.9949, neural stem cells p = 0.0219, ependymal cells p = 0.0208. Data are presented as means ± SEM as appropriate.

Journal: Nature Communications

Article Title: Prdm16 regulates the postnatal fate of embryonic radial glia via Vcam1-dependent mechanisms

doi: 10.1038/s41467-025-60895-y

Figure Lengend Snippet: a Whole-mount preparation, in which the entire surface of the lateral walls of the lateral ventricles is cut out for immunohistochemistry experiments. b Neural stem cells and ependymal cells were identified by a combination of markers on whole-mount preparations. Neural stem cells are positive for Vcam1 and Sox2 with single dots positive for γ-tubulin immunofluorescence (a single basal body associated with a single primary cilium). Ependymal cells are negative for Vcam1 with clusters of γ-tubulin immunofluorescence (multiple basal bodies associated with multiple cilia). Created in BioRender. Zhang, Y. (2025) https://BioRender.com/p63y337 . c An example of a Vcam1 + neural stem cell with a single γ-tubulin + dot (arrowhead) and examples of Vcam1 − ependymal cells with clusters of γ-tubulin + dots (arrows). Scale bar: 10 μm. The experiment was repeated more than three times. d Vcam1, γ-tubulin, and Sox2 immunofluorescence on whole-mount preparations of P35 Prdm16 cKO and control mice. Scale bar: 50 μm. e Quantification of neural stem and ependymal cells at P34–35. n = 3 mice per genotype. Two-tailed Welch’s t -test, total cells p = 0.9949, neural stem cells p = 0.0219, ependymal cells p = 0.0208. Data are presented as means ± SEM as appropriate.

Techniques: Immunohistochemistry, Immunofluorescence, Control, Two Tailed Test

a A diagram shows the design of the BrdU pulse-chase experiment. BrdU was injected 3 times daily between E15.5 and E17.5, and the mice were sacrificed and analyzed at P21. Created in BioRender. Zhang, Y. (2025) https://BioRender.com/z56b814 . b Increased BrdU-labeled cells in the V-SVZs of Prdm16 cKO mice compared with control at P21. The dashed lines delineate V-SVZs. Scale bar: 10 μm. c The quantification of BrdU-labeled cells in the V-SVZ. n = 3 mice per genotype. The black and red dots indicate average results from each mouse and the grey and pink dots indicate the raw counts from each image. Two-tailed Welch’s t -test, p = 0.0431. Data are presented as means ± SEM as appropriate.

Journal: Nature Communications

Article Title: Prdm16 regulates the postnatal fate of embryonic radial glia via Vcam1-dependent mechanisms

doi: 10.1038/s41467-025-60895-y

Figure Lengend Snippet: a A diagram shows the design of the BrdU pulse-chase experiment. BrdU was injected 3 times daily between E15.5 and E17.5, and the mice were sacrificed and analyzed at P21. Created in BioRender. Zhang, Y. (2025) https://BioRender.com/z56b814 . b Increased BrdU-labeled cells in the V-SVZs of Prdm16 cKO mice compared with control at P21. The dashed lines delineate V-SVZs. Scale bar: 10 μm. c The quantification of BrdU-labeled cells in the V-SVZ. n = 3 mice per genotype. The black and red dots indicate average results from each mouse and the grey and pink dots indicate the raw counts from each image. Two-tailed Welch’s t -test, p = 0.0431. Data are presented as means ± SEM as appropriate.

Techniques: Pulse Chase, Injection, Labeling, Control, Two Tailed Test

a V-SVZs from one-month-old mice were used for scRNAseq. UMAP of scRNAseq data. Major cell clusters were identified and color-coded. b Expression of cell-type markers by the major cell types. c The numbers of differentially expressed genes in each cell type. d Prdm16 cKO NSCs upregulated the expression of embryonic radial glia-enriched genes and downregulated the expression of adult NSCs-enriched genes. NES normalized enrichment score. one-sided Kolmogorov–Smirnov (K–S)-like test. In GSEA, the enrichment score is calculated by “walking down” a ranked list of genes, adding a value to a running sum when a gene belongs to the gene set being tested, and subtracting a value when it does not; the final enrichment score is the maximum deviation from zero encountered during this process, essentially reflecting how concentrated the gene from the gene set are at either the top or bottom of the ranked list , . In the left panel, a positive enrichment score indicates that radial glia genes are enriched in the gene set containing genes upregulated in Prdm16 cKO mice. In the right panel, a negative enrichment score indicates that adult NSC genes are enriched in the gene set containing genes downregulated in Prdm16 cKO mice. The black bars in the middle panel represent individual genes. In the left panel, concentration of black bars on the left indicates that radial glia genes are enriched in the gene set containing genes upregulated in Prdm16 cKO mice. In the right panel, concentration of black bars on the right indicates that adult NSC genes are enriched in the gene set containing genes downregulated in Prdm16 cKO mice. e Selected GO terms enriched in genes up and downregulated in Prdm16 cKO NSCs compared with controls.

Journal: Nature Communications

Article Title: Prdm16 regulates the postnatal fate of embryonic radial glia via Vcam1-dependent mechanisms

doi: 10.1038/s41467-025-60895-y

Figure Lengend Snippet: a V-SVZs from one-month-old mice were used for scRNAseq. UMAP of scRNAseq data. Major cell clusters were identified and color-coded. b Expression of cell-type markers by the major cell types. c The numbers of differentially expressed genes in each cell type. d Prdm16 cKO NSCs upregulated the expression of embryonic radial glia-enriched genes and downregulated the expression of adult NSCs-enriched genes. NES normalized enrichment score. one-sided Kolmogorov–Smirnov (K–S)-like test. In GSEA, the enrichment score is calculated by “walking down” a ranked list of genes, adding a value to a running sum when a gene belongs to the gene set being tested, and subtracting a value when it does not; the final enrichment score is the maximum deviation from zero encountered during this process, essentially reflecting how concentrated the gene from the gene set are at either the top or bottom of the ranked list , . In the left panel, a positive enrichment score indicates that radial glia genes are enriched in the gene set containing genes upregulated in Prdm16 cKO mice. In the right panel, a negative enrichment score indicates that adult NSC genes are enriched in the gene set containing genes downregulated in Prdm16 cKO mice. The black bars in the middle panel represent individual genes. In the left panel, concentration of black bars on the left indicates that radial glia genes are enriched in the gene set containing genes upregulated in Prdm16 cKO mice. In the right panel, concentration of black bars on the right indicates that adult NSC genes are enriched in the gene set containing genes downregulated in Prdm16 cKO mice. e Selected GO terms enriched in genes up and downregulated in Prdm16 cKO NSCs compared with controls.

Techniques: Expressing, Concentration Assay

a Numerous DCX + newly born neurons in Prdm16 cKO cerebral cortices at P21. Few new neurons are detected in control cerebral cortex at this age. The dashed lines delineate the boundaries between the white matter and the grey matter. Scale bar: 100 μm. The experiment was repeated more than three times. b A diagram shows the area shown in ( a ) and ( c ) and quantified in ( d ). Created in BioRender. Zhang, Y. (2025) https://BioRender.com/y53n311 . c DCX + newborn neurons alongside GFAP + radial processes in the cerebral cortex of Prdm16 cKO mice at P21. Scale bar: 20 μm. d Quantification of the number of DCX + cells at P21 in the cortex. Only DCX + cells in the grey matter were counted. n = 3 mice per genotype. Two-tailed Welch’s t -test. p = 0.0379. Data are presented as means ± SEM as appropriate. e A diagram shows the design of the postnatal BrdU pulse-chase experiment. BrdU was injected at P14, and the mice were sacrificed and analyzed at P21. Created in BioRender. Zhang, Y. (2025) https://BioRender.com/r15n645 . f Increased DCX + /BrdU + cells in the Prdm16 cKO cortex compared with control at P21. The arrows delineate DCX + /BrdU + cells in the Prdm16 cKO cortex. Scale bar: 50 μm. g Quantification of DCX + /BrdU + cells in the cortex. Only DCX + /BrdU + cells in the grey matter were counted. n = 5 mice per genotype. Two-tailed Welch’s t -test, p = 0.0256. Data are presented as means ± SEM as appropriate.

Journal: Nature Communications

Article Title: Prdm16 regulates the postnatal fate of embryonic radial glia via Vcam1-dependent mechanisms

doi: 10.1038/s41467-025-60895-y

Figure Lengend Snippet: a Numerous DCX + newly born neurons in Prdm16 cKO cerebral cortices at P21. Few new neurons are detected in control cerebral cortex at this age. The dashed lines delineate the boundaries between the white matter and the grey matter. Scale bar: 100 μm. The experiment was repeated more than three times. b A diagram shows the area shown in ( a ) and ( c ) and quantified in ( d ). Created in BioRender. Zhang, Y. (2025) https://BioRender.com/y53n311 . c DCX + newborn neurons alongside GFAP + radial processes in the cerebral cortex of Prdm16 cKO mice at P21. Scale bar: 20 μm. d Quantification of the number of DCX + cells at P21 in the cortex. Only DCX + cells in the grey matter were counted. n = 3 mice per genotype. Two-tailed Welch’s t -test. p = 0.0379. Data are presented as means ± SEM as appropriate. e A diagram shows the design of the postnatal BrdU pulse-chase experiment. BrdU was injected at P14, and the mice were sacrificed and analyzed at P21. Created in BioRender. Zhang, Y. (2025) https://BioRender.com/r15n645 . f Increased DCX + /BrdU + cells in the Prdm16 cKO cortex compared with control at P21. The arrows delineate DCX + /BrdU + cells in the Prdm16 cKO cortex. Scale bar: 50 μm. g Quantification of DCX + /BrdU + cells in the cortex. Only DCX + /BrdU + cells in the grey matter were counted. n = 5 mice per genotype. Two-tailed Welch’s t -test, p = 0.0256. Data are presented as means ± SEM as appropriate.

Techniques: Control, Two Tailed Test, Pulse Chase, Injection

a Increased Vcam1 immunofluorescence in the V-SVZ of juvenile and adult Prdm16 cKO mice. The dashed lines delineate the boundaries between the V-SVZ and the striatum and the boundary between the V-SVZ and the ventricle. Scale bar: 50 μm. b The persistence of GFAP + radial processes is partially rescued in Prdm16-Vcam1 DKO mice. Scale bar: 50 μm. c Whole-mount en-face preparations showing neural stem cells with single γ-tubulin+ dots and ependymal cells with clusters of γ-tubulin+ dots. Scale bar: 50 μm. The cell composition defect in Prdm16 cKO mice is rescued in Prdm16-Vcam1 DKO. d Quantification of Vcam1 immunofluorescence positive area in V-SVZ at P31. n = 3 mice per genotype. Two-tailed Welch’s t -test, p = 0.0287. Data are presented as means ± SEM as appropriate. e Quantification of the number of radial processes at one month of age. Control, n = 4; cKO, n = 4; DCK, n = 8 mice. One-way ANOVA with Tukey’s multiple comparisons test, control vs. Prdm16 cKO, p = 0.002, Prdm16 cKO vs. Prdm16-Vcam1 DKO, p = 0.019. Data are presented as means ± SEM as appropriate. f Quantification of neural stem and ependymal cells at P34–35. Control, n = 3; cKO, n = 3; DCK, n = 4 mice. One-way ANOVA with Tukey’s multiple comparisons test. Ependymal cells, control vs. Prdm16 cKO, p = 0.0017, Prdm16 cKO vs. Prdm16-Vcam1 DKO, p = 0.0097. Neural stem cells, control vs. Prdm16 cKO, p = 0.0124. Prdm16 cKO vs. Prdm16-Vcam1 DKO, p = 0.0262. Data are presented as means ± SEM as appropriate. g The postnatal persistence of newly born DCX + neurons in the cerebral cortex seen in Prdm16 cKO mice is rescued in Prdm16-Vcam1 DKO mice. The dashed lines delineate the boundaries between the white matter and the grey matter. Scale bar: 100 μm. h Quantification of DCX + cells at P21 in the cerebral cortex. Only DCX + cells in the grey matter were counted. n = 3 mice per genotype. One-way ANOVA with Tukey’s multiple comparisons test. Control vs. Prdm16 cKO, p = 0.0041. Prdm16 cKO vs. Prdm16-Vcam1 DKO, p = 0.0055. Data are presented as means ± SEM as appropriate. i Vcam1 mRNA detected by RNAscope at P30. LV lateral ventricle. Scale bar: 50 μm. j Quantification of Vcam1 mRNA levels detected by RNAscope in V-SVZ at P30. n = 3 mice per genotype. Two-tailed Welch’s t -test. p = 0.6004. Data are presented as means ± SEM as appropriate. k Vcam1 mRNA expression level in the NSCs of P34–35 Prdm16 cKO and control mice as determined by scRNA-seq. Wilcoxon test with Bonferroni correction, N.S. not significant.

Journal: Nature Communications

Article Title: Prdm16 regulates the postnatal fate of embryonic radial glia via Vcam1-dependent mechanisms

doi: 10.1038/s41467-025-60895-y

Figure Lengend Snippet: a Increased Vcam1 immunofluorescence in the V-SVZ of juvenile and adult Prdm16 cKO mice. The dashed lines delineate the boundaries between the V-SVZ and the striatum and the boundary between the V-SVZ and the ventricle. Scale bar: 50 μm. b The persistence of GFAP + radial processes is partially rescued in Prdm16-Vcam1 DKO mice. Scale bar: 50 μm. c Whole-mount en-face preparations showing neural stem cells with single γ-tubulin+ dots and ependymal cells with clusters of γ-tubulin+ dots. Scale bar: 50 μm. The cell composition defect in Prdm16 cKO mice is rescued in Prdm16-Vcam1 DKO. d Quantification of Vcam1 immunofluorescence positive area in V-SVZ at P31. n = 3 mice per genotype. Two-tailed Welch’s t -test, p = 0.0287. Data are presented as means ± SEM as appropriate. e Quantification of the number of radial processes at one month of age. Control, n = 4; cKO, n = 4; DCK, n = 8 mice. One-way ANOVA with Tukey’s multiple comparisons test, control vs. Prdm16 cKO, p = 0.002, Prdm16 cKO vs. Prdm16-Vcam1 DKO, p = 0.019. Data are presented as means ± SEM as appropriate. f Quantification of neural stem and ependymal cells at P34–35. Control, n = 3; cKO, n = 3; DCK, n = 4 mice. One-way ANOVA with Tukey’s multiple comparisons test. Ependymal cells, control vs. Prdm16 cKO, p = 0.0017, Prdm16 cKO vs. Prdm16-Vcam1 DKO, p = 0.0097. Neural stem cells, control vs. Prdm16 cKO, p = 0.0124. Prdm16 cKO vs. Prdm16-Vcam1 DKO, p = 0.0262. Data are presented as means ± SEM as appropriate. g The postnatal persistence of newly born DCX + neurons in the cerebral cortex seen in Prdm16 cKO mice is rescued in Prdm16-Vcam1 DKO mice. The dashed lines delineate the boundaries between the white matter and the grey matter. Scale bar: 100 μm. h Quantification of DCX + cells at P21 in the cerebral cortex. Only DCX + cells in the grey matter were counted. n = 3 mice per genotype. One-way ANOVA with Tukey’s multiple comparisons test. Control vs. Prdm16 cKO, p = 0.0041. Prdm16 cKO vs. Prdm16-Vcam1 DKO, p = 0.0055. Data are presented as means ± SEM as appropriate. i Vcam1 mRNA detected by RNAscope at P30. LV lateral ventricle. Scale bar: 50 μm. j Quantification of Vcam1 mRNA levels detected by RNAscope in V-SVZ at P30. n = 3 mice per genotype. Two-tailed Welch’s t -test. p = 0.6004. Data are presented as means ± SEM as appropriate. k Vcam1 mRNA expression level in the NSCs of P34–35 Prdm16 cKO and control mice as determined by scRNA-seq. Wilcoxon test with Bonferroni correction, N.S. not significant.

Techniques: Immunofluorescence, Two Tailed Test, Control, RNAscope, Expressing

a Differentiation of radial glial cells in control and Prdm16 cKO mice. b A diagram shows embryonic slowly dividing radial glia become the adult type B cells postnatally. Vcam1 is highly expressed in the embryonic V-SVZ. In control mice, Prdm16 induces a reduction in Vcam1 levels postnatally, triggering the transition from embryonic radial glia to adult NSCs. In Prdm16 cKO mice, the expression of Vcam1 stays high postnatally, leading to the persistence of radial glia and the continuation of cortical neurogenesis. Created in BioRender. Zhang, Y. (2025) https://BioRender.com/r64e200 .

Journal: Nature Communications

Article Title: Prdm16 regulates the postnatal fate of embryonic radial glia via Vcam1-dependent mechanisms

doi: 10.1038/s41467-025-60895-y

Figure Lengend Snippet: a Differentiation of radial glial cells in control and Prdm16 cKO mice. b A diagram shows embryonic slowly dividing radial glia become the adult type B cells postnatally. Vcam1 is highly expressed in the embryonic V-SVZ. In control mice, Prdm16 induces a reduction in Vcam1 levels postnatally, triggering the transition from embryonic radial glia to adult NSCs. In Prdm16 cKO mice, the expression of Vcam1 stays high postnatally, leading to the persistence of radial glia and the continuation of cortical neurogenesis. Created in BioRender. Zhang, Y. (2025) https://BioRender.com/r64e200 .

Techniques: Control, Expressing

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